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. 2022 Jun 10;13:878037. doi: 10.3389/fpls.2022.878037

Figure 1.

Figure 1

Generation of CmNAC-NOR disrupted mutant lines by CRISPR/Cas9. (A) Illustration of the CmNAC-NOR gene structure and the three gRNAs in exons 1 and 3. (B) Six independent mutations at gRNA3 targeting sites. (C) Ploidy test of transgenic lines. Left, diploid line NOR-g3-22; Middle, tetraploid line NOR-g3-20; and Right, total number of diploid and tetraploid plants obtained. (D) Validated edited lines by Sanger sequencing. The yellow boxes and dotted lines indicate the deleted nucleotides and their position. (E) Amino acid alignment of the CmNAC-NOR gRNA3-target region in VED (WT), nor-3, and nor-1. NAC subdomain A is shown by a dashed green line above the protein sequences.