TABLE 1.
End product formation from cell suspensions of L. lactis NZ9050(pNZ2600)
| Growth conditions | Concn of end product (conversion efficiency [%])a
|
||||
|---|---|---|---|---|---|
| α-AL | Diacetyl | Acetoin | Lactic acid | Acetic acid | |
| Unaerated | 0.9 (9) | 0.3 (3) | 0.1 (1) | 17.2 (86) | 0.0 (0) |
| Aerobic | 5.7 (57) | 1.6 (16) | 0.5 (5) | 0.0 (0) | 4.2 (21) |
a
Cells were incubated in 100 mM potassium phosphate buffer containing 0.5% glucose for 24 h under unaerated and aerobic conditions. Before harvesting, cells were induced for NOX overexpression by the addition of 2 ng of nisin/ml during mid-exponential phase (OD600 = 1.0). Values are expressed in millimolar units produced per 10 mM glucose converted; values in parentheses are percentages of glucose converted. Standard deviations of the concentration data are routinely 0.1 mM.