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. 2021 Nov 2;24(3):323–331. doi: 10.4103/aja202157

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Copyright: ©The Author(s)(2021)

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Comparison of indices of oxidative stress injury in sham group, model group, Tt-SOD 100 U g⁻¹ (low-dose) group, Tt-SOD 300 U g⁻¹ (medium-dose) group, and Tt-SOD 500 U g⁻¹ (high-dose) group. (a) Representative images of DCFH-DA fluorescence staining to assess ROS levels in each group; (b) quantitative analysis (green: DCFH-DA, blue: DAPI, 630× magnification). The levels of (c) CAT, (d) SOD, (e) MDA, (f) GSH-PX, and (g) NO in each group were detected using ELISA. Results are expressed as mean ± standard deviation. ^**P < 0.01, the indicated group versus sham group; ^#P < 0.05 and ^##P < 0.01, the indicated group versus model group. CAT: catalase; DAPI: 4’,6-diamidino-2-phenylindole; DCFH-DA: 2’,7’-dichlorofluorescein diacetate; ELISA: enzyme-linked immunosorbent assays; GSH-PX: glutathione peroxidase; MDA: malondialdehyde; NO: nitric oxide; SOD: superoxide dismutase; Tt-SOD: Thermus thermophilic-superoxide dismutase.