FIGURE 1.

HFD feeding exacerbates cisplatin‐induced kidney damage. Mice were allowed free access to water and standard chow before experiments were initiated. From the start, mice were fed either a high‐fat diet containing 60% fat (HFD) or a low‐fat diet containing 10% fat (LFD) for 14 days. Cisplatin (20 mg/kg body weight) or vehicle (cisplatin‐dissolving solution as a control) was administered to the mice on the 11th day after the change in diet. Kidneys and blood were collected 3 days after those administrations. (a) Kidney sections were subjected to PAS staining. The white arrowhead indicates congested tubules (damage score: 2). The black arrowhead indicates disrupted tubules (damage score: 3). (b) Tubular damage was scored as described in the Materials and Methods. (c) Concentrations of blood urea nitrogen (BUN) in the plasma was determined 3 days after those administrations. Results are expressed as the means ± SEM (n = 4–6).