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. 2022 Jun 22;10(6):e004381. doi: 10.1136/jitc-2021-004381

Figure 5.

Figure 5

Synthesis and characterization of miR-21–3p-conjugated nanoparticles miR-21–3p-AuNp. (A) A schematic view of the design and construction process of miR-21–3p-AuNp. (B) UV-Vis absorption spectra of AuNp and miR-21–3p-AuNp. (C) Hydrodynamic diameter distributions of AuNp and miR-21–3p-AuNp, showing the successful self-assembly process of the nanoengineering miRNA into an auric sphere hybrid system. (D-E) Transmission electron micrograph images (TEM) of Au and miR-21–3p-AuNp. (F) The zeta potential of miR-21–3p-AuNp measured in PBS at pH 7.4. (G-H) Relative mRNA level of miR-21–3p and TXNRD1 after the treatment with miR-21–3p-AuNp or AuNp in melanoma cell. (I) Immunoblotting analysis of TXNRD1 after the treatment with miR-21–3p-AuNp or AuNp in melanoma cell. (J) Relative cell viability of melanoma cells treated with ferroptosis inducer, IFN-γ and miR-21–3p-AuNp or AuNp. Erastin was used at 10 µM in both cell lines. RSL3 was used at 0.5 µM in A375 and 1 µM in A2058 cell line. IFN-γ was used at 50 ng/mL in both cell lines. Data represent the mean±SD of triplicates. P value was calculated by two-tailed Student’s t-test. *P<0.05, **p<0.01, ***p<0.001. AuNp, gold nanoparticles; IFN, interferon; miRNA, microRNA; ns, non-significant; TXNRD1, thioredoxin reductase 1.