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. 2022 Jun 8;15(6):726. doi: 10.3390/ph15060726

Figure 3.

Figure 3

Effect of intrathecal injection of NAN-190 and MDL-72222 on the analgesic effect of [6]-shogaol on oxaliplatin-induced cold (A) and mechanical (B) allodynia. NAN-190 and MDL-72222 are 5-HT1A and 5-HT3 receptor antagonists, respectively. Cold allodynia was assessed by using acetone drop test (A) and mechanical allodynia was measured by using von Frey filament test (B). All groups received oxaliplatin injection four days prior to behavior tests. PBS and 20% DMSO was used as control for NAN-190 and MDL-72222, respectively. Ten percent DMSO or [6]-shogaol was intraperitoneal injected 20 min after intrathecal injection of PBS, 20% DMSO, NAN-190, and MDL-72222. Baseline: before injection of PBS, 10%, and 20% DMSO, NAN-190, MDL-72222, and [6]-shogaol. + 1h: 1 h after injection of PBS, 10 and 20% DMSO, NAN-190, MDL-72222, and [6]-shogaol. Data are presented by mean ± SEM. OXA + PBS + 10% DMSO: n = 5, OXA + PBS + [6]-shogaol: n = 7, OXA + 20% DMSO + [6]-shogaol: n = 7, OXA + NAN-190 + [6]-shogaol: n = 7, OXA + MDL-72222 + [6]-shogaol: n = 6. ** p < 0.01, *** p < 0.001, ****, p < 0.0001 vs. Control with two-way ANOVA followed by Tukey’s multiple comparisons test.