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Optimization of sandwich ELISA system using three different concentrations of SARS-CoV-2 S1 mAbs (capture antibodies) (10, 5 and 2.5 µg/mL) and three concentrations of gold-conjugated nanobodies (AuNPs-Nbs) as detector probes: (A) AuNPs-Nbs concentration of 5 µg/mL, (B) AuNPs-Nbs concentration of 2.5 µg/mL and (C) AuNPs-Nbs concentration of 1.2 µg/mL. NB: The optimum pairing concentrations were obtained by using S1mAb at 5 µg/mL concentration and AuNPs-Nbs at 2.5 µg/mL concentration.
