Table 1.
Anti-AChE activity studied by ITC method and comparison with colorimetric method results. * Reprinted/adapted with permission from Refs. [23,24,25]. 2022, Dominik Szwajgiwer.
| Compound | Abbreviation | Inhibitory Activity | |||
|---|---|---|---|---|---|
| ITC | Ellman’s Assay nmol/L of Eserine * |
||||
| % Inhibition | IC50 (μmol/μmol AChE) |
Ka × 103 (L/mol) | |||
| methyl syringate | MS | 90.93 ± 7.13 | 5.50 ± 0.43 | 154.32 ± 11.12 | 9.1 ±1.6 |
| 4-hydroxyphenylpyruvic acid | x | 84.93 ± 5.82 | 5.89 ± 0.40 | 253.16 ± 14.53 | 23.6 ± 1.5 |
| salicylic acid | SA | 82.30 ± 4.77 | 6.07 ± 0.35 | 51.55 ± 3.77 | 11.8 ± 0.4 |
| 4-hydroxyphenylacetic acid | 4-OH-PA | 80.20 ± 8.04 | 6.24 ± 0.63 | 66.23 ± 5.46 | NA |
| 4-hydroxybenzoic acid | 4-OH-BA | 78.60 ± 8.30 | 6.36 ± 0.67 | 58.48 ± 7.81 | 3.7 ± 0.3 |
| homovanillic acid | HVA | 77.50 ± 2.59 | 6.45 ± 0.22 | 136.05 ± 5.44 | 21.8 ± 0.4 |
| nordihydroguaiaretic acid | NDGA | 77.20 ± 4.84 | 6.47 ± 0.41 | 57.80 ± 4.26 | 37.3 ± 1.2 |
| protocatechuic acid | PCA | 77.15 ± 5.16 | 6.50 ± 0.43 | 57.63 ± 2.58 | NA |
| 3-hydroxybenzoic acid | 3-OH-BA | 74.80 ± 8.63 | 6.68 ± 0.77 | 64.10 ± 6.04 | 2.6 ± 0.1 |
| vanillic acid | VA | 73.70 ± 1.90 | 6.79 ± 0.18 | 52.36 ± 2.53 | 3.2 ± 0.0 |
| syringic acid | SRA | 71.80 ± 3.12 | 6.96 ± 0.30 | 183.15 ± 6.69 | 5.4 ± 0.1 |
| homogentisic acid | HGA | 68.78 ± 0.88 | 7.16 ± 0.09 | 116.92 ± 2.05 | 9.6 ± 2.4 |
| gentisic acid | GNA | 62.38 ± 6.39 | 8.02 ± 0.82 | 76.92 ± 8.78 | 68.6 ± 0.7 |
| gallic acid | GA | 53.70 ± 2.86 | 9.32 ± 0.50 | 51.55 ± 3.57 | 3.8 ± 0.1 |
| ethyl 4-hydroxybenzoate | E-4-OH-BA | 15.93 ± 0.75 | 31.38 ± 1.48 | 20.53 ± 1.79 | 28.1 ± 0.1 |
| ethyl vanillate | EV | 14.60 ± 2.33 | 34.19 ± 5.46 | 38.76 ± 5.91 | 33.5 ± 2.5 |