Table 5.

3³ factorial design—response values (Z-Ave, PDI and ZP) of the independent factors of 27 experiments for lipid nanoparticles containing each liquid lipids tested (data are expressed as mean ± standard deviation).

	Miglyol® 810 N			Microalgae Oil (Schizochytrium)			Halamphora cf. salinicola Crude Oil with Microalgae Oil
Sample No.	Z-Ave 1 (nm)	PDI 2 (-)	ZP 3 (mV)	Z-Ave 1 (nm)	PDI 2 (-)	ZP 3 (mV)	Z-Ave 1 (nm)	PDI 2 (-)	ZP 3 (mV)
1	232.6 ± 5.9	0.166 ± 0.016	53.0 ± 1.4	236.1 ± 1.0	0.158 ± 0.031	51.4 ± 1.4	220.3 ± 3.6	0.193 ± 0.011	50.8 ± 1.3
2	186.8 ± 5.3	0.198 ± 0.018	52.6 ± 0.2	198.8 ± 6.8	0.172 ± 0.019	49.4 ± 2.4	218.9 ± 2.8	0.224 ± 0.011	50.7 ± 1.3
3	145.8 ± 1.6	0.232 ± 0.012	51.1 ± 1.0	178.1 ± 3.0	0.195 ± 0.012	50.7 ± 1.8	211.5 ± 6.5	0.250 ± 0.010	49.5 ± 0.6
4	201.1 ± 4.7	0.155 ± 0.024	51.0 ± 1.3	223.0 ± 4.9	0.185 ± 0.010	50.8 ± 0.6	204.1 ± 0.7	0.181 ± 0.018	50.8 ± 1.1
5	205.2 ± 2.6	0.159 ± 0.013	50.5 ± 0.5	215.3 ± 2.8	0.184 ± 0.008	49.0 ± 1.5	226.0 ± 2.2	0.264 ± 0.013	49.6 ± 1.9
6	155.4 ± 1.8	0.160 ± 0.012	49.1 ± 2.1	173.9 ± 1.3	0.149 ± 0.009	48.9 ± 0.4	177.3 ± 2.9	0.180 ± 0.005	49.4 ± 0.6
7	190.1 ± 2.2	0.119 ± 0.004	50.6 ± 1.0	239.3 ± 5.3	0.163 ± 0.013	51.6 ± 0.1	246.2 ± 4.2	0.194 ± 0.013	53.3 ± 1.8
8	195.0 ± 2.6	0.155 ± 0.007	49.4 ± 0.5	238.2 ± 5.2	0.161 ± 0.018	43.4 ± 1.6	217.9 ± 2.5	0.141 ± 0.019	50.5 ± 0.9
9	151.8 ± 1.7	0.152 ± 0.018	51.5 ± 3.4	199.5 ±3.4	0.158 ± 0.019	46.4 ± 0.8	237.4 ± 4.3	0.247 ± 0.004	50.3 ± 1.4
10	196.7 ± 6.6	0.175 ± 0.006	48.3 ± 1.2	219.6 ±3.5	0.162 ± 0.002	47.5 ± 1.3	194.4 ± 1.3	0.149 ±0.010	53.2 ± 2.2
11	203.6 ± 4.2	0.186 ± 0.010	51.4 ± 0.7	175.5 ± 2.4	0.146 ± 0.010	49.7 ± 0.6	163.1 ± 2.8	0.134 ± 0.016	50.4 ± 0.9
12	156.9 ± 3.0	0.218 ± 0.006	49.7 ± 1.0	170.3 ± 3.3	0.190 ± 0.015	50.0 ± 1.2	172.8 ± 1.7	0.190 ± 0.005	50.5 ± 1.2
13	241.8 ± 5.4	0.160 ± 0.020	51.5 ± 1.3	249.0 ± 3.3	0.169 ± 0.014	49.0 ± 0.9	253.4 ± 1.9	0.187 ± 0.004	44.5 ± 0.5
14	194.5 ± 3.5	0.194 ± 0.014	50.1 ± 0.8	190.0 ± 2.7	0.152 ± 0.012	51.3 ± 1.2	230.2 ± 4.4	0.195 ± 0.008	52.9 ± 1.2
15	179.9 ± 3.2	0.178 ± 0.018	49.9 ± 0.6	171.2 ± 1.6	0.163 ± 0.019	48.3 ± 1.4	175.9 ± 0.4	0.158 ± 0.006	45.9 ± 1.1
16	216.6 ± 3.1	0.122 ± 0.021	52.7 ± 0.6	253.9 ± 3.4	0.134 ± 0.025	51.1 ± 0.6	252.0 ± 0.9	0.172 ± 0.033	50.2 ± 0.4
17	207.2 ± 5.3	0.157 ± 0.010	49.4 ± 1.3	238.3 ± 5.1	0.162 ± 0.031	47.7 ± 1.1	228.9 ± 3.1	0.198 ± 0.010	51.4 ± 1.2
18	179.9 ± 3.1	0.147 ± 0.005	51.2 ± 1.9	171.9 ± 3.3	0.144 ± 0.012	45.7 ± 0.8	248.2 ± 3.9	0.224 ± 0.018	51.3 ± 1.1
19	280.8 ± 6.9	0.180 ± 0.018	53.9 ± 2.3	225.1 ± 5.6	0.135 ± 0.005	50.2 ± 0.7	259.7 ± 5.3	0.209 ± 0.013	49.7 ± 1.7
20	247.4 ± 7.4	0.259 ± 0.006	48.4 ± 1.3	178.0 ± 3.3	0.158 ± 0.015	45.1 ± 3.7	203.0 ± 3.7	0.178 ± 0.009	51.7 ± 0.6
21	209.3 ± 4.4	0.234 ± 0.006	48.7 ± 1.7	180.9 ± 4.1	0.181 ± 0.020	48.8 ± 0.8	203.9 ± 3.6	0.210 ± 0.025	48.9 ± 0.3
22	253.1 ± 9.0	0.129 ± 0.018	53.1 ± 0.6	249.5 ± 2.7	0.157 ± 0.012	51.3 ± 0.8	241.4 ± 4.1	0.150 ± 0.017	49.9 ± 1.6
23	195.2 ± 3.4	0.155 ± 0.015	50.8 ± 0.5	202.0 ± 1.9	0.153 ± 0.016	50.3 ± 0.8	189.9 ± 1.4	0.175 ± 0.010	43.4 ± 0.1
24	189.5 ± 4.3	0.210 ± 0.009	47.9 ± 1.8	174.2 ± 5.2	0.183 ± 0.018	47.3 ± 2.6	211.6 ± 1.7	0.195 ± 0.023	46.8 ± 0.6
25	245.7 ± 3.3	0.124 ± 0.021	53.1 ± 1.4	263.7 ± 5.8	0.137 ± 0.041	51.7 ± 0.5	272.2 ± 4.7	0.158 ± 0.016	49.6 ± 1.5
26	215.4 ± 4.3	0.178 ± 0.005	48.5 ± 1.0	225.0 ± 2.1	0.149 ± 0.024	47.8 ± 3.0	209.5 ± 3.1	0.178 ± 0.014	49.0 ± 2.3
27	173.1 ± 3.0	0.163 ± 0.011	49.9 ± 1.6	194.2 ± 3.4	0.144 ± 0.010	44.1 ± 1.2	190.6 ± 2.3	0.170 ± 0.031	51.4 ± 1.3

¹ Z-Ave = mean particle size; ² PDI = polydispersity index; ³ ZP = zeta potential (pH of solutions within the range 6.2–6.5); RI = refractive index = 1.45.