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. 2022 Jun 20;27(12):3949. doi: 10.3390/molecules27123949

Figure 2.

Figure 2

Viability of A549 cells measured by WST-1 assay after incubation with eluates from (A) test specimens A (15 mm × 0.5 mm), (B) test specimens B (50 mm × 1.0 mm) or (C) test specimens C (50 mm × 3.0 mm) of different compositions (OC, Orthocryl®; OCLC, Orthocryl® LC; E, Loctite® EA 9483; PP, polypropylene) and previous watering conditions. The test specimens were stored in distilled water at room temperature for 0, 48 or 168 h followed by a 24 h storage in cell culture medium (DMEM, Dulbecco’s Modified Eagle Medium) under shaking conditions. The DMEM eluates were used undiluted (100%) or diluted (50, 25, 12.5, 6.25, 3.125%) for a 24 h incubation of A549 cells followed by determination of cell viability using the WST-1 assay. Percentages represent comparison with cells treated with DMEM vehicle control (set to 100%). The data are mean values ± SEM of n = 3–4 per group. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 vs. DMEM control; one-way ANOVA with Dunnett’s post hoc test. The dashed red line shows the 30% deviation of the treatment groups from the control value (DMEM group).