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. 2022 Jun 15;14(12):2473. doi: 10.3390/nu14122473

Figure 3.

Figure 3

Apoptosis of RPE cells detected by TUNEL assay. (A–C) C57BL/6 mice were gavaged saline (100 μL/10 g) for seven days (n = 4). (D–F) Firstly, C57BL/6 mice were gavaged DMSO (1%) for three days, and then they were subretinally injected 7KCh. After injection, gavaging with DMSO (1%) continued for four days. (G–I) C57BL/6 mice were gavaged saline (100 μL/10 g) for three days, then they were subretinal injected 7KCh. After injection, gavaging with saline (100 μL/10 g) continued for four days. (J–L) C57BL/6 mice were gavaged GW3965 (10 mg/kg) for three days, and then they were subretinally injected 7KCh. After injection, gavaging with GW3965 (10 mg/kg) continued for four days. Retinas were harvested, sectioned, and subjected to TUNEL analyses. Blue represents nuclei, and the green represents apoptosis cells. Scale bar 50 μm. (M) Fluorescent intensity was measured at eight different locations and averaged. Data are represented as mean ± SEM of three independent experiments. Differences of p < 0.05 were considered statistically significant.