Figure 6.

ROS levels of ARPE-19 cells. (A) As a control, ARPE-19 cells were cultured with a serum-free medium for 48 h. (B) ARPE-19 cells were incubated with DMSO (0.02%) for 24 h, followed by 7KCh (20 μM) for 24 h. (C) ARPE-19 cells were cultured with serum-free medium for 24 h and then incubated with 7KCh (20 μM) for 24 h. (D) ARPE-19 cells were incubated with GW3965 (2 μM) for 24 h, followed by 7KCh (20 μM) for 24 h. The apoptosis of ARPE-19 cells was measured by ROS detection. Scale bar 100 μm. (E) DCF fluorescence intensity was quantified with Image J. Data are represented as mean ± SEM of three independent experiments. Differences with p < 0.05 were considered statistically significant.