Figure 8.

Flow cytometric analysis of mitochondrial membrane potential in ARPE-19 cells. (A) As a control, ARPE-19 cells were cultured with a serum-free medium for 48 h. (B) ARPE-19 cells were incubated with DMSO (0.02%) for 24 h, followed by 7KCh (20 μM) for 24 h. (C) ARPE-19 cells were cultured with serum-free medium for 24 h and then incubated with 7KCh (20 μM) for 24 h. (D) ARPE-19 cells were incubated with GW3965 (2 μM) for 24 h, followed by 7KCh (20 μM) for 24 h. (E) ARPE-19 cells were first transfected with siLXRα as previously described and then incubated with 7KCh (20 μM) and GW3965 (2 μM) for 24 h. (F) ARPE-19 cells were first transfected with siLXRβ as previously described and then incubated with 7KCh (20 μM) and GW3965 (2 μM) for 24 h. After treatment, the cells were collected and subjected to flow cytometric analysis of mitochondrial membrane potential. (G) Mitochondrial membrane potential was quantified. Data are represented as mean ± SEM of three independent experiments. Differences of p < 0.05 were considered statistically significant.