Skip to main content
. 2022 Jun 15;14(12):2473. doi: 10.3390/nu14122473

Figure 9.

Figure 9

Western blot of proteins in ARPE-19 cells. (A) As a control, ARPE-19 cells were cultured with a serum-free medium for 48 h. (DMSO) ARPE-19 cells were incubated with DMSO (0.02%) for 24 h, followed by 7KCh (20 μM) for 24 h. (7KCh) ARPE-19 cells were cultured with serum-free medium for 24 h and then incubated with 7KCh (20 μM) for 24 h. (GW3965) ARPE-19 cells were incubated with GW3965 (2 μM) for 24 h, followed by 7KCh (20 μM) for 24 h. (siLXRα) ARPE-19 cells were first transfected with siLXRα as previously described and then incubated with 7KCh (20 μM) and GW3965 (2 μM) for 24 h. (siLXRβ) ARPE-19 cells were first transfected with siLXRβ as previously described and then incubated with 7KCh (20 μM) and GW3965 (2 μM) for 24 h. Total protein was collected from the cultured ARPE-19 cells. (B) P62 expression levels in ARPE-19 cells. (C) LC3I/II expression levels in ARPE-19 cells. (D) mTOR expression levels in ARPE-19 cells. Data are represented as mean ± SEM of three independent experiments. Differences with p < 0.05 were considered statistically significant.