Figure 2.

Treatment with capsaicin affects the expression of NF-kB regulated genes. (A–D) Capsaicin treatment decreased the mRNA expression levels of IL6, TNFα, IL-1β and NLRP3 as compared to infected tissues. Values were normalized to GAPDH as housekeeping gene for indicated genes. Control (CON), H. pylori infected (HP), H. pylori infected + Capsaicin treated (HP + Caps). (E) Immunoblot showing the expression of phospho NF-κB and NF-κB after capsaicin treatment in H. pylori infected mice. Experiments were performed in triplicates. α Tubulin was used as internal control. Densitometry of representative blot was performed. Data are represented as mean ± SEM. Values were tested by ANOVA followed by Tukey’s post hoc test. ‘*’and ‘**’ represent significant differences between groups at p < 0.05, p < 0.01, respectively.