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. 2022 May 24;10(6):1082. doi: 10.3390/microorganisms10061082

Figure 2.

Figure 2

Biofilm formation inhibition: reduction of C. difficile VCC in dual-species biofilms compared to single-species (CD) biofilms at different ratios and incubation durations. Washed biofilms were scraped and resuspended in 1 mL of PBS before plating 100 µL of dilutions on BHI for single-species CD biofilms or on BHI supplemented with 0.03 µg/mL of amphotericin B for dual-species (CD + SB) biofilms to determine the number of CFU. Means were calculated from at least four different biological replicates. Asterisks indicate statistical differences of C. difficile viability with the single-species (CD) biofilm determined by a Mann–Whitney test (* p ≤ 0.05).