(A–D). Bacterial load of MtbΔ2159 at 5 and 10 week post infected lung and spleen. (A). Lung bacillary load of wild type H37Rv, MtbΔ2159, and CΔ2159 strains at 5 week post-infection. (B). Spleen bacillary load at 5 week post-infection of wild type H37Rv, MtbΔ2159, and CΔ2159 strains. (C). Lung bacillary load of wild type H37Rv, MtbΔ2159, and CΔ2159 strains at 10 week post-infection. (D). Spleen bacillary load of wild type H37Rv, MtbΔ2159, and CΔ2159 strains at 10 week post-infection. The graphs were plotted with the mean ± standard deviation of five independent animals in each group. Statistical analysis was performed using Two-way ANOVA with Bonferroni post-test correction. *** p < 0.001 and ** p < 0.01.