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DNA alignment of BG1F primer, BG1R primer reverse complement, and their 138-bp target sequences in the Rhs family gene. The accession numbers of the target sequences in NCBI database are shown in brackets after the strains names. Dots (∙) indicate identical nucleotides aligned at the same site as the BG1 primers; letters A, T, G, and C indicate different nucleotides aligned at the same site.
