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. 2022 Jun 15;10(6):1223. doi: 10.3390/microorganisms10061223

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Profiles of SYBR Green real-time quantitative PCR (qPCR) assays on seed-carrying bacterial strains Burkholderia glumae Os48 (A,C,E) and B. gladioli Os50 (B,D,F). (A,B) Amplification curves of SYBR Green qPCR assays. Numbers 1, 2, 3, and 4 indicate the 1-μL templates from seed-carrying bacteria at the concentration of 2 × 10⁵, 2 × 10⁴, 2 × 10³, and 2 × 10² CFU·seed⁻¹, respectively; 5 is the template from ultrapure water-treated seeds. (C,D) Melting curves of SYBR Green qPCR assays show the single specific peak at Tm of 83 °C. (E,F) Standard curves of SYBR Green qPCR assays show a linear response between bacterial cell concentration (lg CFU·seed⁻¹) and cycle threshold.