Figure 8.
Profiles of EvaGreen droplet digital PCR (dPCR) assays on seed-carrying bacterial strains Burkholderia glumae Os48 (A,C) and B. gladioli Os50 (B,D). (A,B) Fluorescence amplitude 1-D plot of EvaGreen droplet dPCR assays on seed-carrying bacteria at the 10-fold diluted concentrations from 2 × 105 to 2 × 100 CFU·seed−1; aqueous solutions from mock inoculation with ultrapure water were used as the no template control. Blue line shows the threshold separating negative droplets from positive droplets; blue dots indicate positive droplets of amplification; grey dots indicate negative droplets of no amplification. One positive droplet (<2) detected at the concentration of 2 × 101 CFU·seed−1 was not considered as positive. (C,D) Regression curves of EvaGreen droplet dPCR show a linear response between bacterial cell concentration (lg CFU·seed−1) and copy number of detected target DNA.