FIG. 3.

Binding of hybrid flagella to proteins immobilized on plastic was determined by ELISA using anti-H7 flagellum antibodies and secondary antibodies. Binding in increasing concentrations (FliC at 0 to 1.25 μg/ml) of bihybrid flagella from E. coli BFS1 (—●—) and D1,D2,D3/FliCΔ (—○—), YadA84-385/FliCΔ (—▾—), and FliCΔ (—▿—) flagella to human plasma fibronectin (A), to type IV collagen (B) immobilized at a constant concentration of 2 pmol per well, and to fetuin (25 μg/ml) (C). Note the dose-dependent, saturable binding of bihybrid flagella to fibronectin as well as to type IV collagen.