TABLE 2.
Transformation frequencies of R. solanacearum recipient strains with transgenic plant DNA and plasmids containing T-DNA
| Donor DNA | T-DNA/plant genomea | Transformation frequency for R. solanacearum strainb:
|
|
|---|---|---|---|
| GMI1000 | GMI1000FB3 | ||
| Plasmid pZpop1 | 3.76 × 10−7 ± 2.1 × 10−8 | ||
| Lycopersicon esculentum Zpop1 | 2 | <1.53 × 10−9 | |
| Nicotiana tabacum Zpop1-EP1 | 1 | <1.6 × 10−9 | |
| Nicotiana tabacum Zpop1-EP2 | 4 | <1.36 × 10−9 | |
| Plasmid pKHG3 | 1.43 × 10−7 ± 5.3 × 10−8 | ||
| Lycopersicon esculentum pKHG3 | 1 | <4.38 × 10−9 | |
| Nicotiana tabacum pKHG3-ER1 | 1 | <3.2 × 10−9 | |
| Nicotiana tabacum pKHG3-ER2 | 3 | <2.91 × 10−9 | |
| Plasmid pBin19 | 8.9 × 10−8 ± 2.3 × 10−8 | ||
| Nicotiana tabacum pBin19-ER1 | 1 | <1.95 × 10−9 | |
| Nicotiana tabacum pBin19-ER2 | 3 | <2.35 × 10−9 | |
a
The genome sizes of Lycopersicon esculentum and Nicotiana tabacum are 1 × 109 and 4.6 × 109 bp, respectively.
b
The length of sequence similarity of each side of the marker mutation between the donor DNA and the recipient strain is 719–907 bp for GMI1000 and 700–600 bp for GMI1000FB3.