TABLE 1.
Host organism | UV spectraa | D. vulgaris Miyazaki F Abb | N terminusc | Mw | pI |
---|---|---|---|---|---|
D. vulgaris Miyazaki F | 409, 419, 523, 552 | + | APKAPADGLKM | 14,000 | 10.6 |
MR-1(pRKM3F) | 409, 419, 523, 552 | + | AAPKAPADGLKM | 14,000 | Alkaline |
S. oneidensis MR-1 | 408, 419, 522, 551 | − | ADQKLSDFHAES | 12,000 | 5.8 |
The first value is the peak in the oxidized state, and the other values are the peaks in the dithionite-reduced state.
Interaction with antibody (Ab) against D. vulgaris Miyazaki F.
The N-terminal sequences were determined with a Shimadzu PPSQ-10 protein sequencer coupled to a Shimadzu C-R7A analyzer.