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Gene layout of the two commonly used reporter molecules in NGS and some of the typical primers employed in their sequencing. (a) The 16S rRNA gene, used for sequencing of bacterial and archaeal communities. The variable regions allow for the delineation between OTUs, while the conservative regions are used as universal primer binding sites. (b) The ITS regions flanking the 5.8S rRNA gene used for annotating eukaryotes.
