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. 2022 Jun 16;11(6):1182. doi: 10.3390/antiox11061182

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Representative chromatograms (measured at 0 mV potential) of BH₄ in the brain, heart and kidney of control and LPS-treated septic rats. (B) Concentration levels in these tissues as calculated from BH₄ standard curve. Data are mean ± SD of the number of indicated independent measurements. Note: * means significantly different to control rats. *, p-value is < 0.05; **, p-value is < 0.01 to indicate significance against the control. (C) Representative chromatograms (measured at 0 mV potential) of BH₄ in the heart and brain of control rats with and without spiking with authentic BH₄ standard.