Figure 5.

Analysis of cell-cycle phase distribution after 24 and 48 h of incubation with IC₅₀ concentrations in RKO (a) and SW480 (c) cell lines. Flow cytometry histograms of the FL2 red channel reading fluorescence of RKO (b) and SW480 (d) cells incubated with IC₅₀ concentrations of Ru5 and Ru7 complexes for 48 h and stained with propidium iodide. Results were expressed as mean ± SD of at least three independent experiments. Results were statistically different from the negative control for * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.