Table 1.
Ingredients (g kg−1) |
Experimental Diets (g Protein kg−1 Diet) | Control Diet (450 g Protein kg−1 Diet) |
||
---|---|---|---|---|
250 | 300 | 350 | ||
Fish meal | 90 | 270 | 270 | 450 |
Soybean meal | 330 | 230 | 330 | 330 |
Yellow corn | 280 | 250 | 205 | 90 |
Wheat bran | 195 | 155 | 100 | 45 |
Fish oil | 60 | 50 | 50 | 40 |
Cholesterol | 5 | 5 | 5 | 5 |
Di-calcium phosphate | 20 | 20 | 20 | 20 |
Vitamin and minerals 1 | 20 | 20 | 20 | 20 |
Proximate analysis (g kg−1) | ||||
Dry matter | 893.5 | 899.6 | 901.8 | 906 |
Crude protein | 256.2 | 319.6 | 352.1 | 451.7 |
Crude lipid | 71.8 | 92.6 | 89.3 | 81.2 |
Ash content | 78.1 | 81.2 | 90.1 | 112.3 |
Crude fiber | 49.8 | 39.3 | 39.8 | 33.8 |
Nitrogen-free extract 2 | 544.1 | 467.3 | 428.7 | 321 |
Gross energy (MJ kg−1 diet) 3 | 18.70 | 19.22 | 19.20 | 19.38 |
1 Vitamin premix (mg or IU kg−1 diet): vitamin A, 6000 IU; vitamin D3, 2000 IU; ascorbic acid, 200 mg; vitamin E, 50 mg; menadione, 5 mg; thiamine, 15 mg; riboflavin, 15 mg; nicotinic acid, 30 mg; pantothenic acid, 35 mg; pyridoxine HCl, 6 mg; cyanocobalamin, 0.03 mg; biotin, 0.2 mg; inositol, 200 mg; folic acid, 3 mg; iodine, 0.4 mg; cobalt, 0.1 mg; copper, 4 mg; iron, 150 mg; zinc, 80 mg; manganese, 20 mg; selenium, 0.1 mg; magnesium, 100 mg. 2 Nitrogen-free extract (%) = 100 − (crude protein + ether extract + crude fiber + ash). 3 Gross energy (MJ kg−1) = (crude protein × 23.6 + ether extract × 39.5 + Nitrogen-free extract × 17.2)/100.