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. 2022 May 27;15(6):676. doi: 10.3390/ph15060676

Figure 2.

Figure 2

MRM chromatograms for MPI8 (transitions m/z 601.4→545.3 and 601.4→157.2): Not-fully-separated multiple peaks on a Kinetex F5 column (A) and an Acquity HSS-T3 column (B); distinctly separated double peak chromatograms of MPI8 on a Synergi Fusion-RP column (C); single peak with good peak shape on a Kinetex HILIC column (D). The time program of the gradient: (A,B,D) Phase B was initially kept at 5% for 0.5 min, increased from 5% to 90% in the next 2.5 min, then decreased to 5% in 1.0 min, and kept stably at 5% for 1 min; (C) Phase B was initially at 20% for 0.5 min, increased from 20% to 90% in 4.5 min, kept at 90% for 2 min, decreased to initial concentration (20%) in 1 min and equilibrated for 2 min.