Table 4.

Stability results of warfarin and hydroxywarfarins enantiomers in rat plasma under different conditions.

Conditions	Levels (ng/mL)	Autosampler for 24 h (n = 6)		Room Temperature for 8 h (n = 6)		Three Treeze-Thaw Cycles (n = 6)		–70 °C for 30 Days (n = 6)
		RR (%)	CV (%)	RR (%)	CV (%)	RR (%)	CV (%)	RR (%)	CV (%)
R-10(R)-OH-warfarin	2	95.39	4.72	99.11	3.55	103.79	8.30	98.40	7.59
	600	100.54	6.12	91.58	1.25	104.35	6.67	102.11	7.06
S-10(R)-OH-warfarin	2	103.93	5.40	98.12	7.05	94.89	9.15	96.43	5.76
	600	102.06	3.97	102.23	2.84	101.28	4.40	98.04	5.98
R-7-OH- warfarin	2	107.75	4.04	95.76	3.25	110.48	5.79	107.27	2.47
	600	106.83	5.98	90.41	8.14	103.49	3.39	101.79	5.20
S-7-OH- warfarin	2	94.13	2.38	103.91	3.81	103.36	7.14	101.46	9.92
	600	103.37	6.40	98.94	16.25	103.39	6.57	100.22	2.55
R-warfarin	20	100.99	15.7	103.34	10.87	100.52	11.26	94.53	6.01
	6000	104.10	4.32	94.12	2.38	100.28	10.60	97.23	10.63
S-warfarin	20	100.59	5.88	98.19	10.79	94.13	12.61	91.99	8.42
	6000	94.69	2.54	101.83	4.73	98.50	4.24	99.77	4.72

CV, coefficient of variation; RR, relative recovery.