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. 2022 Jun 7;14(6):1240. doi: 10.3390/v14061240

Figure 2.

Figure 2

Purification, DNA extraction, and structural proteins of TCUP2199. (a) Block gradient of CsCl. The band indicates phage TCUP2199 particles. (b) Estimation of TCUP2199 DNA size by pulsed-field gel electrophoresis. Lanes: M, midrange I PFG markers; second lane indicates uncut TCUP2199 DNA. (c) SDS polyacrylamide gel electrophoresis (10%) of TCUP2199 virion proteins. Purified TCUP2199 particles were boiled in sample buffer (100 mM Tris-HCl, pH 6.8, 4% SDS, 0.2% bromophenol blue, 20% glycerol, 200 mM dithiothreitol) (10 µL) and loaded onto the well.