TABLE 5.
NADH peroxidase activity in various Lactobacillus strains grown in MRS broth at 30°C in the presence or absence of hydrogen peroxide (0.75 mmol/liter)
Organism | Mean sp act (μmol of NADH oxidized/min/mg of total protein)a ± SD
|
||
---|---|---|---|
No hydrogen peroxide (control) | Hydrogen peroxide (0.75 mM) added | No hydrogen peroxideb | |
L. plantarum | 0.143 ± 0.012C | 0.233 ± 0.031A | 0.159 ± 0.018B |
L. paracasei | 0.093 ± 0.020C | 0.241 ± 0.023A | 0.119 ± 0.031B |
Lactobacillus sp. strain 3 | 0.217 ± 0.012C | 0.324 ± 0.021A | 0.215 ± 0.030B |
L. rhamnosus | 0.051 ± 0.029C | 0.347 ± 0.018A | 0.104 ± 0.007B |
L. fermentum | 0.062 ± 0.003C | 0.149 ± 0.021A | 0.052 ± 0.009B |
The values given are the mean of three separate analyses ± the standard deviation. The superscript capital letters indicate the levels of significance (P = 0.05) between treatments and not between organisms (based on Duncan's multiple range test).
In this case, cells were grown in MRS broth containing 0.75 mM H2O2 until late log phase and then transferred to fresh media for ∼18 h to stationary phase without hydrogen peroxide.