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. 2022 Jun 10;14(12):2413. doi: 10.3390/nu14122413

Figure 4.

Figure 4

Resveratrol (RSV) induces apoptosis in A549 cells. Cells were treated with various concentrations of RSV for 48 h. (A) Annexin V-FITC/PI staining was used to detect the proportion of early apoptotic cells, late apoptotic and necrotic cells, and normal cells; (B) TUNEL-FITC/PI staining was used to detect the percentage of apoptosis cells; (C) Apoptosome observed under TEM; (D) Transcriptional expression of BAX and BCL-2 as determined by qRT-PCR. ACTB was used as an internal control; (E) Protein expression of BAX and BCL-2 as determined by Western blot. ACTB was used as an internal control; (F) QRT-PCR analysis of mRNA level of BAX and BCL-2 in the cells treated with or without 80 μM RSV or 30 μM Z-VAD-FMK. ACTB was used as an internal control; (G) Western blot assay of protein levels of BAX and BCL-2 in the cells treated with or without 80 μM RSV or 30 μM Z-VAD-FMK. ACTB was used as an internal control. The data are presented as the mean ± SD of triplicate tests. * p < 0.05 and ** p < 0.01 compared with the control group. ## p < 0.01 compared with 80 μM RSV group.