TABLE 2.
Mass balance of benzo[a]pyrene radiocarbon during a 14-day incubation period
| Day | Mass balance (%)
|
Total recovery (%) | ||||
|---|---|---|---|---|---|---|
| Biomass-associated 14Ca | 14C-aqueous metabolites | Suspended [7-14C]benzo[a]pyreneb | Attached [7-14C]benzo[a]pyrenec | 14CO2 | ||
| Diesel fuel added | ||||||
| 0 | NDd | ND | 0.4 ± 0.1 | 98.5 ± 4.1 | ND | 98.9 ± 4.0 |
| 4 | 1.6 ± 1.5 | 0.2 ± <0.1 | 26.4 ± 1.9 | 70.0 ± 1.5 | 0.3 ± 0.1 | 98.5 ± 1.9 |
| 7 | 16.0 ± 3.8 | 3.0 ± 0.6 | 25.4 ± 13.3 | 29.5 ± 1.0 | 25.4 ± 2.5 | 99.3 ± 7.3 |
| 14 | 8.8 ± 2.0 | 2.6 ± 0.3 | 7.3 ± 1.0 | 17.8 ± 2.8 | 51.0 ± 3.3 | 87.4 ± 1.9 |
| No diesel fuel added | ||||||
| 14e | 2.1 ± <0.1 | 0.5 ± <0.1 | 14.6 ± 8.8 | 82.2 ± 12.3 | 2.8 ± 0.5 | 102.7 ± 3.9 |
a
14C in biomass or in transformation products which were associated with biomass.
b
14C trapped on filters, extracted, and determined to be 14C in [7-14C]benzo[a]pyrene by TLC.
c
More than 95% of the attached 14C was determined to be 14C in [7-14C]benzo[a]pyrene by TLC.
d
ND, not detected (below the detection limit).
e
The negative control consisted of benzo[a]pyrene and inoculum with no diesel fuel added.