Figure 7.

Eriodictyol promoted the expressions of CAT and GPx1 via Nrf2 cascade. (A) Western blot assay demonstrated that supplementation with eriodictyol (5–20 µM; 24 h) enhanced the expressions of CAT and GPx1 in BJ cells following H₂O₂ treatment (500 µM; 1 h). The upregulation of these H₂O₂-removal enzymes is possibly due to the activation of the Nrf2 pathway, as observed by an increase in the expression levels of Nrf-2 and its downstream target HO-1 and a decrease in Keap1 protein expression. GAPDH was used as a loading control. DMSO (0.5%) was used as a vehicle control. Results are representative of three independent experiments. Full unedited blots are shown in Supplementary Figure S2. (B–F). The band intensity of certain proteins from Western blot analysis was quantified with Image J software and normalized to loading control GAPDH. Data represent mean ± SEM of protein expression with respect to untreated control (n = 3). * p < 0.05 versus vehicle controls; ^† p < 0.05 versus H₂O₂-treated cells.