Skip to main content
. 2022 Jun 6;10(6):1160. doi: 10.3390/microorganisms10061160

Figure 1.

Figure 1

Protein purification and substrate specificities of DrRuvC. (A) Size exclusion chromatogram (Superdex 75 10/300 GL column) of recombinant DrRuvC protein. SDS-PAGE of representative eluted fractions is shown in inset. (B) Bandshift analysis of Holliday junctions (HJ-0X and HJ-12X). (C) Bandshift analysis of the indicated DNA structures. From left to right, the binding reactions contained 0, 50, 100, 200, 300, 400, 500 nM of DrRuvC protein. (D) Native PAGE analysis of DrRuvC cleavage of the indicated DNA structures.