Table 1.
Virucidal activity of TTO against FCoVII and HCoV-OC43.
| Formulation (TTO%, EtOH%) |
Time of Exposure (min) |
FCoVII (Log TCID50/mL) |
HCoV-OC43 (Log RNA Copies/mL) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Virus Exposed to TTO |
Virus Control | RV | Virus Inactivation (%) |
Virus Exposed to TTO |
Virus Control | RV | Virus Inactivation (%) |
||
| A * (3.33, 5.33) |
5 | <0.50 | 4.00 | ≥3.50 | ≥99.97 * | 2.58 | 3.36 | 0.78 | 83.41 |
| 15 | <0.50 | 4.00 | ≥3.50 | ≥99.97 * | 2.56 | 3.28 | 0.72 | 80.96 | |
| 30 | <0.50 | 4.00 | ≥3.50 | ≥99.97 * | 2.78 | 4.16 | 1.38 | 95.83 | |
| B (0.66, 1.06) |
5 | 3.75 | 3.50 | 0.00 | 0.00 | 3.79 | 3.36 | 0.00 | 0.00 |
| 15 | 3.75 | 4.00 | 0.25 | 43.76 | 3.69 | 3.28 | 0.00 | 0.00 | |
| 30 | 4.00 | 4.50 | 0.50 | 68.36 | 4.78 | 5.13 | 0.35 | 55.36 | |
| C (0.13, 0.21) |
5 | 3.50 | 3.50 | 0.00 | 0.00 | 3.24 | 3.36 | 0.00 | 0.00 |
| 15 | 4.00 | 4.00 | 0.00 | 0.00 | 3.45 | 3.28 | 0.00 | 0.00 | |
| 30 | 4.50 | 4.50 | 0.00 | 0.00 | 4.80 | 5.13 | 0.33 | 53.28 | |
| D (0.00, 5.33) |
5 | ND | ND | ND | ND | ND | ND | ND | ND |
| 15 | ND | ND | ND | ND | ND | ND | ND | ND | |
| 30 | 4.00 | 4.00 | 0.00 | 0.00 | 4.12 | 4.05 | 0.00 | 0.00 | |
| Disinfectant ** | 5 | <0.50 | 3.50 | ≥3.00 | ≥99.90 * | 1.90 | 4.03 | 2.13 | 99.26 |
Reduction value (RV) is the reduction of the virus in test product (formulations A–D) compared to the virus control not exposed to the test product. The virus percentage inactivation is calculated using the formula 1 − (1/10RV) × 100%. * Due to cytotoxicity of the tested formulation A, the detection limit did not allow for the detection of higher FCoVII reduction. ** Disinfectant composition: isazone, benzalkonium chloride 50%, chlorhexidine gluconate, and isopropanol. ND = not determined.