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. Author manuscript; available in PMC: 2022 Jun 24.
Published in final edited form as: Cell Rep. 2016 Jul 7;16(3):781–792. doi: 10.1016/j.celrep.2016.06.042

Figure 4. Genomic-wide Transcriptional Profiling of VCSTO-iAs.

Figure 4.

(A) Heatmap presentation of microarray analysis of MEFs, VCSTO-iAs, and mouse pAs. Genes upregulated in iAs and pAs, compared to MEFs, are shown in red, whereas genes downregulated in iAs and pAs, compared to MEFs, are shown in blue.

(B) GO terms for genes upregulated in both iAs and pAs, relative to MEFs, are shown in red, whereas GO terms associated with genes downregulated in iAs and pAs are shown in blue. The x axis represents enrichment scores, with p value calculated via Fisher’s exact test.

(C) Heatmap presentation of a selected set of fibroblast-related genes (the upper four genes) and astrocyte-related genes (the lower four genes).

(D and E) Real-time PCR validation of the expression of four fibroblast-associated genes (D) and four astrocyte-associated genes (E). The expression in MEFs was defined as 1. n = 3 experimental repeats.

(F) Schematic presentation of different regions of the brain.

(G)Relative expression of regional subtype markers in iAs measured by real-time PCR. DB, dorsal brain; FB, forebrain; HB, hindbrain; VB, ventral brain. n = 3 experimental repeats.

See also Figure S4.