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. 2022 Jun 7;11(12):1526. doi: 10.3390/plants11121526

Figure 5.

Figure 5

Expression profiles of LSU genes of wheat, Arabidopsis and tomato under S deficiency. The mRNA levels of the LSU gene family were measured by RT-qPCR in leaves (A) and roots (B) of each species grown for 3 weeks in a liquid medium under control and S-deficiency conditions. The expression levels were normalized with the ADAPTOR PROTEIN-4-MU-ADAPTIN gene (AT4G24550) in the case of Arabidopsis [46], TIP4I-like family protein (TIP4I, SGN-U584254) for tomato [47] and the Ubiquitin-conjugating enzyme gene (TraesCS4A02G414200) for wheat [48]. Each treatment was performed using three independent experiments. The bars represent the arithmetic mean, and the asterisks indicate the mean of the measurements that are significantly different according to a Student’s t-test with p < 0.05.