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Mode (A) and mean (B) size distributions of pooled sEV fractions by all methods. In both mean and mode size analysis, UC + SEC particles were significantly larger than those obtained by the UC + SEC + UF and SEC + UF methods (UC + SEC + UF, n = 3; all other methods, n = 4; error bars ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001). The mean size of particles obtained by the SEC + UC method were significantly larger than the UC + SEC + UF and SEC + UF methods.
