Figure 5.

Fucoidan detection inside the hydrogel and fucoidan release from the hydrogel. The protocol from Yamazaki and colleagues [34] was adapted to detect fucoidan inside a hydrogel. (a) The SYBR Gold nucleic acid stain allowed detecting different fucoidan amounts inside the hydrogel. (b) Hydrogels with and without fucoidan were incubated in PBS for 6 days. The fucoidan content inside the hydrogel was detected on day 0, 2, and 6 using SYBR Gold. Experiments were repeated three times (n = 3). Significances compared to the control (hydrogel with 0 µg/mL Fucoidan) were calculated with the unpaired t-test (* p < 0.05, ** p < 0.01).