Figure 6.

Biocompatibility of human mesenchymal stem cells (MSC) with injectable chitosan-collagen hydrogels containing different fucoidan concentrations. (a) MSC were cultured on top of the hydrogels (2D culture), (b) MSC were encapsulated into the hydrogels (3D culture). Vital (green) and dead (red) 2D- and 3D-cultured cells were visualized on day two and six using CalceinAM and propidium iodide staining, respectively. Scale bars = 200 µm. (c) MSC were cultivated on hydrogels containing 100 µg/mL fucoidan and visualized by scanning electron microscopy after six days. MSC in black frames numbered with 1 and 2 are shown in a higher magnification in the middle and on the left hand side. Scale bars = 10 µm.