Figure 1.

tsC510G-US28∆ replicates to similar titers as tsC510G in lytically infected fibroblasts. NuFF-1 cells were infected (moi = 0.01 TCID₅₀/cell) as indicated at the (A) permissive (32.5 °C) or (B) non-permissive (39.5 °C) temperature. Extracellular virus was collected over a 16 d time course and titered on naïve NuFF-1 cells at the permissive temperature (32.5 °C) by TCID₅₀. Error bars indicate standard deviation, and statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparison test: (A) * p < 0.05 (WT vs. tsC510G; WT vs. tsC510G-US28∆); ** p < 0.01 (WT vs. tsC510G; WT vs. tsC510G-US28∆); all other comparisons were not significant. (B) * p = 0.0203 (WT vs. tsC510G; WT vs. tsC510G-US28∆); ** p = 0.0169 (WT vs. tsC510G; WT vs. tsC510G-US28∆); day 8: p = 0.0869 (for each, WT vs. tsC510G; WT vs. tsC510G-US28∆); day 16: p = 0.0765 (WT vs. tsC510G) and p = 0.0764 (WT vs. tsC510G-US28∆). LOD, level of detection. n = 3; representative experiment is shown, with 3 technical replicates per time point.