. 2022 Jun 12;14(6):1280. doi: 10.3390/v14061280

Table 1.

Oligonucleotides used in this study.

Primer Use	Sequence (5′ to 3′)	Primer Name
Kan-I-SceI insertion (C510G mutation)	CGCTGCCACCCCCGTGGACCTGTTGGGCGCTCTCAACCTGGGCCTGCCCCTGATGCAAAAGTCGATTTATTCAACAAAGCCACG ¹	IE2 C510G I-SceI 5′
Kan-I-SceI insertion (C510G mutation)	ACCATGACCTGTTTGGGAAACTTTTGCATCAGGGGCAGGCCCAGGTTGAGAGCGCCCAACACGCGTATATCTGGCCCGTACATCG ¹	IE2 C510G I-SceI 3′
sequencing primers	GTGACACATCCACCCGAAGTGGCGCAGCGC	C510G US
sequencing primers	GTCTTCGGGAGGGGTCTCGGTGGGCTGCTC	C510G DS
galK insertion (US28Δ)	GGTGCGTGGACCAGACGGCGTCCATGCACCGAGGGCAGAACTGGTGCTATCCCTGTTGACAATTAATCATCGGCA ²	US28Δ galK 5′
galK insertion (US28Δ)	AGAGGGGCGGACACGGGGTTTGTATGAAAAGGCCGAGGTAGCGCTTTTTTATCAGCACTGTCCTGCTCCTT ²	US28Δ galK 3′
ds oligo	CAGACGGCGTCCATGCACCGAGGGCAGAACTGGTGCTATCTAAAAAAGCGCTACCTCGGCCTTTTCATACAAACCCCGTG	US28Δ ds oligo
sequencing primers	CCGCACATCTATTTTTGCTAATTGC	US28 fwr
sequencing primers	GCGACGAAACCCACCGTCACGG	US28 rev

¹ Underlined sequences are complementary to the pEPKanS vector. ² Underlined sequences are complementary to the galK template, pGalK.