Figure 4. Abdominopelvic CONV and FLASH irradiation in combination with αPD-1 therapy enhance intratumoral CD8+ infiltration and decrease the Treg to T effector ratio in the ID8 ovarian tumor microenvironment.
Flow cytometry analysis for lymphocytes (CD45+), T cells (TCRβ+), CD4+, CD8+, and Treg (CD4+ FoxP3+) infiltration, Ki-67+ proliferation, and CD107a+ staining in the tumor bearing omentum at 96 hours and 17 days post irradiation (n= 5 mice per group). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared by one-way ANOVA followed by Tukey’s multiple comparisons post-hoc test. Error bars represent standard deviation of the mean. At least 2 independent experiments showed similar results.