Table 1.
Comparison of the results of the alkaline and the neutral comet assay. DNA damage was shown as a median of the percentage of DNA in comet tail with an interquartile range (parentheses). Significant differences (p < 0.0001) compared with control (*).
| Cancer Cell Line | Compound | Alkaline Version | Neutral Version |
|---|---|---|---|
| HCT 116 | Control | 1.3 (0.5–2.1) | 2.9 (1.9–4) |
| MM129 IC50 | 10.7 * (4.7–28.1) | 19.4 * (13.3–29.2) | |
| MM129 2 × IC50 | 24.9 * (12.4–46.3) | 26.9 * (19.9–34.3) | |
| MM130 IC50 | 22.7 * (10.3–44) | 21.2 * (14.9–31.7) | |
| MM130 2 × IC50 | 25.6 * (14.1–41.3) | 30.6 * (24.8–36.1) | |
| MM131 IC50 | 31.5 * (19.8–51.3) | 15.8 * (11.7–23.1) | |
| MM131 2 × IC50 | 38.8 * (25.3–57) | 25.6 * (19.7–33.1) | |
| PC-3 | Control | 3.4 (2.2–4.8) | 1.3 * (0.4–2.3) |
| MM129 IC50 | 13.8 * (4–30.6) | 2.4 * (1.1–3.8) | |
| MM129 2 × IC50 | 21.8 * (10.4–38.2) | 7.3 * (5.1–10.3) | |
| MM130 IC50 | 12.4 * (4.2–33.4) | 1.5 * (0.4–3.1) | |
| MM130 2 × IC50 | 19.8 * (9.6–34) | 2.2 * (1.2–3.7) | |
| MM131 IC50 | 29.4 * (17.9–41) | 2.3 * (1.3–3.6) | |
| MM131 2 × IC50 | 40.3 * (28–53.2) | 4.3 * (2.8–6.3) | |
| BxPC-3 | Control | 1.6 * (0.5–2.6) | 2.5 * (0.4–4.4) |
| MM129 IC50 | 6.7 * (2.3–20.7) | 8.7 * (6.1–13.9) | |
| MM129 2 × IC50 | 20.7 * (10.5–27.4) | 10 * (6.9–13.9) | |
| MM130 IC50 | 9.1 * (2.2–25) | 7 * (4.7–12.2) | |
| MM130 2 × IC50 | 15.8 * (4.5–29.9) | 8.5 * (6.1–12.9) | |
| MM131 IC50 | 7.5 * (1.7–23.9) | 9.6 * (6.8–13.4) | |
| MM131 2 × IC50 | 17.4 * (2.5–27.7) | 9.7 * (7.4–12.9) |