TABLE 2.
Specific activities of pectin-depolymerizing enzymes during growth on glucose, polygalacturonate (DE, 0%), and pectin (DE, 90%)a
Carbon source | Sp act (mU/mg)
|
|||||
---|---|---|---|---|---|---|
PEL
|
PAL
|
PG
|
||||
Intracellular | Extracellular | Intracellular | Extracellular | Intracellular | Extracellular | |
Glucose | NDb | ND | ND | ND | ND | ND |
Polygalacturonate | 160 ± 4.0 | 846 ± 31 | 167 ± 3.8 | 615 ± 19 | 0.44 ± 0.02 | 4.48 ± 0.12 |
Pectin | 181 ± 4.5 | 673 ± 25 | 126 ± 3.0 | 673 ± 22 | 0.33 ± 0.01 | 3.45 ± 0.11 |
Cells were grown on MI medium containing a C source at a concentration of 1% and were disrupted as described in Materials and Methods. Intracellular and extracellular fractions were prepared as described in Materials and Methods.
ND, not detected.