TABLE 3.
Specific activities of enzymes related to methanol metabolism during growth on methanol, polygalacturonate (DE, 0%), pectin (DE, 90%), and methanola
| Carbon source | Sp act
|
|||
|---|---|---|---|---|
| AOD (U/mg) | DHAS (mU/mg) | FLD (mU/mg) | FDH (mU/mg) | |
| Polygalacturonate | 0.03 ± 0.01 | 71 ± 3.1 | 14 ± 0.1 | 8 ± 0.1 |
| Pectin | 0.26 ± 0.02 | 94 ± 3.4 | 17 ± 0.2 | 50 ± 0.4 |
| Methanol | 1.12 ± 0.09 | 210 ± 7.3 | 201 ± 2.2 | 129 ± 0.9 |
| Glucose | NDb | ND | 11 ± 0.1 | 10 ± 0.1 |
Cells were grown on MI medium containing a C source at a concentration of 1% and were disrupted as described in Materials and Methods.
ND, not detected.