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Effects of CIN on the tau hyperphosphorylation in vivo. (A–D) The protein levels of phosphorylated tau at thr181, thr205 and Ser396 sites in rat hippocampus were detected by Western blot. n = 8. (E) IHC staining of rat brain immunostained with antibody against thr205-phosphorylated tau (scale bar, hippocampus group: 500 µm; CA1, CA3 group: 250 µm). ## p < 0.01 compared with the control group; * p < 0.05, ** p < 0.01 compared with the model group.
