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. 2022 Jun 5;10(6):1159. doi: 10.3390/microorganisms10061159

Figure 2.

Figure 2

Validation of dcas9 expression at mRNA and protein levels (A) Transcription analysis of dcas9 by qRT-PCR in transformants of M. gallisepticum (Mga) and M. hominis (Mho). Quantitative dcas9 expression was normalized to the expression level of tuf for both species. Boxplot was plotted from three repeated experiments (three independent transformants and three technical repeats for each). (B,C) dCas9 protein level by Western blot for wild-type M. gallisepticum, M. hominis and pRLM5L2-dcas9 transformants.