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. 2022 Jun 5;10(6):1159. doi: 10.3390/microorganisms10061159

Figure 4.

Figure 4

Efficiency of genes knockdown via CRISPRi in two mycoplasma species. Expression changes of target-genes (fur, whiA, spxA and hup1 for M. gallisepticum and deoA for M. hominis) in transformants expressing dCas9 and corresponding sgRNAs compared to the wild-type mycoplasma. dCas9–transformants that express dCas9 and don’t express sgRNAs; sg1–constructed sgRNA1, sg2–constructed sgRNA2. The sequences of each designed sgRNAs and position of target sequences can be found in Table 1, Supplementary Figure S4. (*: p-value < 0.05; ns: not significant).